ATCC56-X.2 MITC-STO (ATCC 56-X)
|资源名称||MITC-STO (ATCC 56-X)|
|种属||Mus musculus, mouse embryo|
|应用领域||Mitomycin C-treated cells are used as a feeder layer|
|培养基||The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.|
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
|存储条件||liquid nitrogen vapor phase;Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO|
Storage Temperature: Liquid nitrogen vapor phase
|描述||These cells are generated from mouse embryonic fibroblasts, STO cell line (ATCC CRL-1503), by a treatment of Mitomycin C. |
These cells are provided to be used as feeder cells to support the growth of other cells. They have been treated with Mitomycin C and will not replicate. The cells will begin to deteriorate in 2 to 3 weeks after plating. Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. Such populations are employed for maintenance of embryonal stem cells such as ES-D3 (ATCC CRL-1934) or teratocarcinoma stem cells (see ATCC CRL-1535 and ATCC CRL-1566) in the undifferentiated state.
It is recommended that the feeder cells be plated 24 hours before use at 6 x 106/T-75 or 2 x 106/T-25 in order to obtain a 100% confluent monolayer for stem cells growth. Feeder layers are also employed to enhance the growth at low density populations of many hybridomas, colon carcinoma cell line (ATCC CCL-247) and squamous cell carcinoma cell lines (ATCC CRL-1624 and ATCC CRL-1629). This action is due partly to conditioning of the substrate and the medium. A feeder layer confluence of 30 % is adequate and can be obtained by plating 2 x 106/T-75.