上海11选5走势图

主页 > 美国ATCC > 干细胞
价格:请临时咨询
数量:
ATCCSCRC-1040.1 MEF (CF-1) IRR
基本信息
资源编号 ATCCSCRC-1040.1
资源名称 MEF (CF-1) IRR
种属 Mus musculus, mouse Embryo
类型 Fibroblast
形态 Fibroblast
提供形式 frozen
安全等级 1
应用领域 Irradiated cells for use as feeder layer
培养方法
培养基 The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
传代方法 Medium Renewal: Twice a week or when pH decreases
生长条件 Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
生长特性 adherent
详细说明
描述 These cells have been growth-arrested by irradiation with 10,00 rads. The cells will begin to deteriorate 7 to 10 days after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 7 to 10 days.
Age 14 days gestation embryo
Gender male and female mixed
Strain CF-1, non-inbred mouse strain (non-agouti albino) from Carworth Farms
Clinical Data male and female mixed
Year of Origin 2004
必发彩票开户 湖北11选5 上海11选5走势图 云南11选5走势图 云南11选5开奖 云南11选5走势图 必发彩票投注 湖北11选5开奖 金砖彩票官网 云南11选5走势图