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ATCC50358 2358
基本信息
资源编号 ATCC50358
资源名称 2358
种属 Bodo saliens Larsen and Patterson
分离基物 sediment, No Name Canyon, 2043 feet, Atlantic Ocean, 1992
提供形式 frozen
安全等级 1
模式菌株 no
培养方法
培养基 Medium 1525: Seawater 802 medium
生长条件 Temperature: 25.0°C
Duration: grown with Enterobacter aerogenes ATCC 13048 and mixed bacteria
存储条件 Cryoprotective SolutionDMSO 2.0 mlFresh growth medium w/o bacteria 8.0 ml1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat. 2. Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min.3. Adjust the concentration of cells at least 2 x 106/ml in freshmedium.4. Mix the cell preparation and the cryoprotective solution in equal portions. 5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.) 7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.8. To establish a culture from the frozen state place the vial in a 35°C water bath. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial. Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 ml ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC庐 700831).9.     Incubate at 25°C with the cap screwed on tightly.10.   Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 ml to 10.0 ml of bacterized ATCC medium 1525.11.   Follow the protocol for maintenance of culture.
详细说明
Year of Origin 1992
Cross References Nucleotide (GenBank) AF174379 18S ribosomal RNA gene, partial sequence
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