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ATCC30874 CCAP 1518/1a
基本信息
资源编号 ATCC30874
资源名称 CCAP 1518/1a
种属
Deposited As Naegleria gruberi Schardinger
分离基物 pre-1950
提供形式 dried
安全等级 1
模式菌株 no
培养方法
培养基 Medium 997: Fresh water ameba medium
传代方法 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
生长条件 Temperature: 25.0°C
Duration: grown with Escherichia coli ATCC 11775
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
存储条件 1. Allow the cells to encyst. To detach cysts from the plate flush the surface with 5 ml fresh ATCC medium 1323 (Page's Balanced Salt Solution). Rub the surface of the plate with a spread bar to detach adhering amoebae.2. Transfer the cyst suspension to a sterile centrifuge tube. 3. If the cyst concentration does not exceed 2 x 106 cysts/ml adjust the suspension to that concentration. To adjust the concentration, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield 2 x 106. 4. While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows: Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath. Allow the DMSO to solidify. Add the required volume of refrigerated medium. Dissolve the DMSO by inverting the tube several times.
详细说明
描述 Zymodeme Gr-4
CCAP 15181/a is no longer available for CCAP
Maximum temperature tolerance 37C
genetic cluster M
Genetic cluster M [RF34321] = Naegleria gruberi cluster 3 [RF34247].
Equivalent to CCAP 1518/1a, Group I intron in SSUrRNA gene
Thermal ecology
biochemical identification
Year of Origin 1950
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