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ATCCPCS-210-010 Primary Subcutaneous Pre-adipocytes; ..
基本信息
资源编号 ATCCPCS-210-010
资源名称 Primary Subcutaneous Pre-adipocytes; ..
种属 Homo sapiens, human Mature adipose tissue
形态 Spindle-shaped, fibroblast-like
提供形式 frozen 1 mL
安全等级 1
应用领域 Adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, creation of iPS cell lines
培养方法
培养基 Obtain one Fibroblast Growth Kit-Low Serum from the freezer; make sure that the caps of all components are tight. Thaw the components of the growth kit just prior to adding them to the basal medium.  Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer. Table 1. Fibroblast Growth Kit-Low Serum Components (ATCC® PCS-201-041)
生长条件 Component Volume Final Concentration
rh FGF β 0.5 mL 5 ng/mL
L-glutamine 18.75 mL 7.5 mM
Ascorbic acid 0.5 mL 50 μg/mL
Hydrocortisone hemisuccinate 0.5 mL 1 μg/mL
rh Insulin 0.5 mL 5 μg/mL
Fetal Bovine Serum 10.0 mL 2% Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of either of the optional components (GA solution or PSA solution) to be added to the complete growth media is summarized in Table 2. Table 2. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)
Component Volume Final Concentration
Gentamicin-Amphotericin B Solution 0.5 mL Gentamicin: 10 µg/mL
Amphotericin B: 0.25 µg/mL
Penicillin-Streptomycin-Amphotericin B Solution 0.5 mL Penicillin: 10 Units/mL
Streptomycin: 10 µg/mL
Amphotericin B: 25 ng/mL
Phenol Red 0.5 mL 33 µM Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for 30 days.
生长特性 Adherent
详细说明
描述 ATCC® Human Pre-Adipocytes are cryopreserved at the second passage to ensure the highest viability and plating efficiency. ATCC® Primary Cell Solutions™ cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability.
Age Adult
Gender Lot-specific
Ethnicity Lot-specific
Volume 1 mL
Cells per Vial One vial contains a minimum of 1.0 x 106 viable cells
Sterility Tests Negative for bacteria, yeast, fungi
Negative for Mycoplasma
Viral Testing Negative for HIV-1, HIV-2, Hepatitis-B and Hepatitis-C.
C of A Certificate of Analysis
FAQs PCS-210-010 Pre-adipocytesThe PCS-210-010 cells are derived from white adipose tissue.  It is very difficult to obtain brown adipose tissue from an adult.Date Updated: 12/27/2013
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