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ATCCPCS-210-010 Primary Subcutaneous Pre-adipocytes; ..
基本信息
资源编号 ATCCPCS-210-010
资源名称 Primary Subcutaneous Pre-adipocytes; ..
种属 Homo sapiens, human Mature adipose tissue
形态 Spindle-shaped, fibroblast-like
提供形式 frozen 1 mL
安全等级 1
应用领域 Adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, creation of iPS cell lines
培养方法
培养基 Obtain one Fibroblast Growth Kit-Low Serum from the freezer; make sure that the caps of all components are tight. Thaw the components of the growth kit just prior to adding them to the basal medium.  Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer. Table 1. Fibroblast Growth Kit-Low Serum Components (ATCC® PCS-201-041)